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rabbit anti pac1r  (Alomone Labs)


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    Structured Review

    Alomone Labs rabbit anti pac1r
    Rabbit Anti Pac1r, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+pac1r/Anti-PACAP+Receptor+1+Antibody/pm37511603-438-15-21
    Average 93 stars, based on 8 article reviews
    rabbit anti pac1r - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Pituitary adenylate cyclase-activating polypeptide receptor activation in the hypothalamus recruits unique signaling pathways involved in energy homeostasis.
    Article Snippet: .. PAC1R antibody specificity was validated by preincubating rabbit anti-PAC1R with PAC1R blocking peptide (No. BLP-VR003; Alomone Labs, Jerusalem, Israel). .. Following visualization of PAC1R signal alone, blots were stripped and reprobed in an identical fashion for b-actin using a mouse anti-b-actin (No. MA5-15452; 1:2,000; Thermo Fisher Scientific, Rockford, IL) and HRP-conjugated antimouse (No. 7076P2; 1:5,000; Cell Signaling Technologies, Danvers, MA).

    Article Title: Viral-Mediated Knockdown of Nucleus Accumbens Shell PAC1 Receptor Promotes Excessive Alcohol Drinking in Alcohol-Preferring Rats
    Article Snippet: .. Sections were then incubated in a blocking solution for 1 h (5% normal goat serum, 0.2% Triton X-100 in TBS) and then incubated in primary antibodies in blocking solution for 48 hr at 4 °C (rabbit anti-PAC1R, AVR-003, 1:250, Alomone Labs, Jerusalem, Israel; chicken anti-GFP, ab13970, 1:1,000, Abcam, Cambridge, MA; for immunizing peptide validation see Supplementary Figure 1 in . .. After TBS washes, secondary antibody incubation (anti-rabbit AF555, A21429 1:500, Invitrogen, Carlsbad, CA; anti-chicken AF488, 103-545-155 1:500, Jackson ImmunoResearch, West Grove, PA) was performed for 2 h at room temperature.

    Incubation:

    Article Title: Viral-Mediated Knockdown of Nucleus Accumbens Shell PAC1 Receptor Promotes Excessive Alcohol Drinking in Alcohol-Preferring Rats
    Article Snippet: .. Sections were then incubated in a blocking solution for 1 h (5% normal goat serum, 0.2% Triton X-100 in TBS) and then incubated in primary antibodies in blocking solution for 48 hr at 4 °C (rabbit anti-PAC1R, AVR-003, 1:250, Alomone Labs, Jerusalem, Israel; chicken anti-GFP, ab13970, 1:1,000, Abcam, Cambridge, MA; for immunizing peptide validation see Supplementary Figure 1 in . .. After TBS washes, secondary antibody incubation (anti-rabbit AF555, A21429 1:500, Invitrogen, Carlsbad, CA; anti-chicken AF488, 103-545-155 1:500, Jackson ImmunoResearch, West Grove, PA) was performed for 2 h at room temperature.

    Article Title: Downregulation of PACAP and the PAC1 Receptor in the Basal Ganglia, Substantia Nigra and Centrally Projecting Edinger–Westphal Nucleus in the Rotenone model of Parkinson’s Disease
    Article Snippet: After 4 × 15 min 0.1M PBS washes, sections were permeabilized by 0.5% Triton X-100 (Sigma-Aldrich Kft, Budapest, Hungary) in PBS and subsequently treated with 2% normal donkey serum (NDS, Jackson Immunoresearch, Suffolk, UK) diluted in PBS for 60 min. .. The specimens were incubated overnight at room temperature in the cocktail of the primary antisera: rabbit anti-PAC1R (1:500, Cat. No.: AVR-003, Alomone Laboratories, Jerusalem, Israel, lot # AN0102) and mouse monoclonal anti-tyrosine-hydroxylase (TH, 1:1000, Cat. No.: T2928 Sigma-Aldrich, Hungary) diluted in PBS containing 2% NDS. .. After 2 × 15 min washes in PBS, the sections were placed into a mixture of secondary antibodies containing cyanine 3 (Cy3)-conjugated donkey anti-rabbit (1:500; Cat. No: 711-165-152 Jackson Immunoresearch Europe, Ely, UK) and Alexa-488-labeled donkey anti-mouse (1:400, Cat. No.: 706-545-148 Jackson) sera for three hours in PBS.

    Article Title: Downregulation of PACAP and the PAC1 Receptor in the Basal Ganglia, Substantia Nigra and Centrally Projecting Edinger-Westphal Nucleus in the Rotenone model of Parkinson's Disease.
    Article Snippet: After 4 × 15 min 0.1M PBS washes, sections were permeabilized by 0.5% Triton X-100 (Sigma-Aldrich Kft, Budapest, Hungary) in PBS and subsequently treated with 2% normal donkey serum (NDS, Jackson Immunoresearch, Suffolk, UK) diluted in PBS for 60 min. .. The specimens were incubated overnight at room temperature in the cocktail of the primary antisera: rabbit anti-PAC1R (1:500, Cat. No.: AVR-003, Alomone Laboratories, Jerusalem, Israel, lot # AN0102) and mouse monoclonal anti-tyrosine-hydroxylase (TH, 1:1000, Cat. No.: T2928 Sigma-Aldrich, Hungary) diluted in PBS containing 2% NDS. .. After 2 × 15 min washes in PBS, the sections were placed into a mixture of secondary antibodies containing cyanine 3 (Cy3)-conjugated donkey anti-rabbit (1:500; Cat. No: 711-165-152 Jackson Immunoresearch Europe, Ely, UK) and Alexa-488-labeled donkey anti-mouse (1:400, Cat. No.: 706-545-148 Jackson) sera for three hours in PBS.

    Biomarker Discovery:

    Article Title: Viral-Mediated Knockdown of Nucleus Accumbens Shell PAC1 Receptor Promotes Excessive Alcohol Drinking in Alcohol-Preferring Rats
    Article Snippet: .. Sections were then incubated in a blocking solution for 1 h (5% normal goat serum, 0.2% Triton X-100 in TBS) and then incubated in primary antibodies in blocking solution for 48 hr at 4 °C (rabbit anti-PAC1R, AVR-003, 1:250, Alomone Labs, Jerusalem, Israel; chicken anti-GFP, ab13970, 1:1,000, Abcam, Cambridge, MA; for immunizing peptide validation see Supplementary Figure 1 in . .. After TBS washes, secondary antibody incubation (anti-rabbit AF555, A21429 1:500, Invitrogen, Carlsbad, CA; anti-chicken AF488, 103-545-155 1:500, Jackson ImmunoResearch, West Grove, PA) was performed for 2 h at room temperature.



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